Coding

Part:BBa_K1882000:Design

Designed by: Milena Krach   Group: iGEM16_Tuebingen   (2016-10-14)


Ketohexokinase


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 314


Design Notes

The codon usage of this part was optimized to balance DNA synthesis and expression in saccaromyces cerevisiae. Therefore longterm and shortterm repeats had to be modified and biobrick restriction sites were removed.The part was modified for RFC25 and RFC10 at the prefix and suffix of the part.


Source

rattus norvegicus (UniProt KB - Q02974)

References